Margarita V. Kustova, Elena A. Muzyko, Yakov V. Tivon, Valentina N. Perfilova, Valery A. Kataev, Guzel M. Latypova, Ivan N. Tyurenkov, Victor S. Sirotenko

Effect of solid herbal extract of Primula veris L. on the psychoemotional state of rats after chronic alcohol intoxication

Introduction

Effect of solid herbal extract of primula veris l. On the psychoemotional state of rats after chronic alcohol intoxication. Investigates Primula veris L. extract's effect on rats' psychoemotional state post-chronic alcohol intoxication, finding anxiolytic and antidepressant benefits. Compares effects with phenotropil and mildronate.

Abstract

Introduction: The aim of the study was the effect of solid herbal extract of Primula veris L. and comparison drugs phenotropil and mildronate on the psycho-emotional state of rats after chronic alcohol intoxication (CAI). Materials and Methods: CAI was modeled by replacing drinking water with a 10% solution of ethyl alcohol with sucrose (50 g/L) for 6 months. After alcoholization, rats at the age of 16 months were divided into the following groups: 1 – intact – animals without CAI received oral 0.9% sodium chloride solution for 14 days; 2 – control – rats after ethanol withdrawal, which were injected with a 0.9% sodium chloride solution in a similar regimen; 3, 4 and 5 – experimental – females after CAI received oral solid herbal extract of Primula veris L. at a dose of 30 mg/kg, phenotropil 25 mg/kg, mildronate 50 mg/kg, respectively. After the end of treatment, to assess the psycho-emotional state of the animals, the Open field test, Elevated plus maze test, Marble burying test and Porsolt forced swim test were performed. Results and Discussion: In animals after CAI, increased anxiety is observed, which manifested itself in a greater number of stands with support, urinations and boluses in the Open field test compared to the intact group. In the Elevated plus maze test, the trend remained the same: rats in the control group entered the open compartment installation less often, hung from it less often, and spent less time in the open compartment. In addition, females after CAI exhibited compulsive behavior in the Marble burying test. In the Porsolt forced swim test, animals in the control group had a shorter period of active swimming compared to intact females and a longer period of immobilization, which indicates depressive behavior in the former. Solid herbal extract of Primula veris L., phenotropil and mildronate contributed to the improvement of the psycho-emotional state of animals after CAI. Conclusion: Thus, solid herbal extract of Primula veris L. at a dose of 30 mg/kg, which was administered orally once a day to female rats for 14 days after the withdrawal of ethanol, has a pronounced anxiolytic and antidepressant effect, while phenotropil and mildronate (25 mg/kg and 50 mg/kg, respectively, in a similar regimen) had a greater anticompulsive effect.

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