Robestri Natalia Sitanggang

COMPARISON OF 10% BUFFERED FORMALIN NEUTRAL FIXATION SOLUTION WITH BOUIN ON MICROSCOPIC IMAGES OF CHICKEN HEPAR AND CEREBRUM WITH HEMATOXYLIN EOSIN (HE) STAINING

Introduction

Comparison of 10% buffered formalin neutral fixation solution with bouin on microscopic images of chicken hepar and cerebrum with hematoxylin eosin (he) staining. Compare 10% Buffered Formalin Neutral (NBF) and Bouin fixation for chicken hepar and cerebrum tissue microscopy using HE staining. NBF showed superior microscopic images for histopathology.

Abstract

The fixation process is an important stage in the manufacture of histopathology preparations. Fixation aims to prevent autolysis and tissue degradation, so that the results can be observed both anatomically and microscopically. Fixation solutions that are often used in routine histopathological examinations are 10% Formalin Buffer Neutral and Bouin. This study was used to determine the results of the comparison of 10% NBF fixation solution with Bouin on the microscopic picture of hepar and cerebrum with hematoxylin eosin staining. The type of research used is a cross sectional approach with a qualitative descriptive design. Sampling was performed on chicken animals. The samples used were hepar and cerebrum fixed with 10% NBF and 10% Bouin for 12 hours, stained with Hematoxylin Eosin and observed microscopically. Then give an assessment with good, less good, or not good categories based on assessment indicators based on cell color and shape. The results showed a microscopic picture of hepatic and cerebrum tissue as much as 6 preparations fixed with 10% NBF liquid showed good results. While hepatic and cerebrum tissue fixed with bouin liquid as many as 6 preparations showed poor results. The conclusion is that there are differences in the microscopic results of hepatic and cerebrum tissue fixed with 10% NBF solution and Bouin. Good microscopic observation results based on the assessment indicator criteria are tissues fixed with 10% NBF liquid.

Review

This study addresses a critical aspect of histopathology, focusing on the comparative efficacy of two commonly used fixation solutions, 10% Buffered Neutral Formalin (NBF) and Bouin's solution, in preserving the microscopic morphology of chicken hepar and cerebrum tissues. The objective is clearly stated: to determine which fixative yields superior microscopic images when stained with Hematoxylin and Eosin (H&E). This foundational research is relevant to ensuring high-quality tissue preparations, which are paramount for accurate diagnosis and research in pathology. The methodology employs a cross-sectional, qualitative descriptive design, which is suitable for an initial comparative assessment. The study fixed chicken hepar and cerebrum samples for 12 hours with both NBF and Bouin's solution, followed by H&E staining and microscopic observation. Assessment relied on subjective categories of "good," "less good," or "not good" based on cell color and shape. While this provides a basic framework, the abstract could benefit from further detail on the quantitative aspects of the "6 preparations" for each fixative (e.g., how many unique samples, replicates, or fields of view). More importantly, the robustness of the assessment could be enhanced by specifying criteria for each category, incorporating blinded evaluation by multiple observers, or employing a semi-quantitative scoring system for features like nuclear detail, cytoplasmic preservation, and artifact presence. The findings strongly suggest that 10% NBF significantly outperforms Bouin's solution, with all NBF-fixed preparations showing "good" results compared to "poor" results for all Bouin's-fixed tissues. This conclusion reinforces the established role of NBF as a gold standard in many histopathology laboratories. To strengthen the overall impact and scientific rigor of the work, future iterations or a full manuscript should include illustrative photomicrographs demonstrating the observed differences. Additionally, incorporating a discussion of the underlying chemical principles and potential artifacts associated with each fixative (e.g., Bouin's known to cause picric acid artifacts or tissue shrinkage) would provide valuable context. Expanding the assessment beyond qualitative descriptors to include more objective measurements of cellular integrity or artifact prevalence would further solidify these important findings.

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